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dapi nuclear staining  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology dapi nuclear staining
    Dapi Nuclear Staining, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1459 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining/DAPI/pm42869916-314-14-20
    Average 96 stars, based on 1459 article reviews
    dapi nuclear staining - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Staining:

    Article Title: ETC-159, an Upstream Wnt inhibitor, Induces Tumour Necrosis via Modulation of Angiogenesis in Osteosarcoma
    Article Snippet: FFPE sections were stained with H&E and photographed using a TS2 microscope with a DS-L4-Fi3 camera system from Nikon SBIC-NIC at Biopolis, Singapore at 40× magnification. .. For DAPI staining, the sections were stained with UltraCruz Mounting Medium (Santa Cruz, Dallas, TX, USA) and photographed using an Olympus BX61 with a CoolSNAP HQ2 camera at 40× magnification. ..

    Article Title: Telomeric lncRNA TERRA localizes to stress granules in human ALT cells
    Article Snippet: For imaging, we used Nikon AX laser scanning confocal microscope with Plan Apo λ 60x Oil objective and Nikon Eclipse Ti2 microscope equipped with the CREST Optics X-Light V2 Spinning Disk module with 100X/1.4 NA oil objective. .. For immunofluorescence, in addition to the smiFISH protocol, after the post-hybridization washes and before DAPI staining, cells were blocked for 1.5 hours with 2% BSA in PBS with 0.1% Triton-X (PBT) and subsequently incubated for 1.5 hours with primary antibodies (1:250 mouse anti-G3BP1 (sc-365338, Santa Cruz Biotechnology); 1:250 mouse anti-FUS (sc-47711, Santa Cruz Biotechnology); 1:500 mouse anti-p-histone H2A (Ser139) (05-636, Merck); 1:500 rabbit anti-TERF2IP (anti-RAP1) (NB100-292, Novus Biologicals)) washed 5 times with PBT, incubated for 1.5 hours with secondary antibodies (1:1500 goat anti-mouse conjugated with Alexa488, A28175 or 1:1500 goat anti-rabbit conjugated with Alexa647, A21245), and again washed 5 times with PBT. ..

    Article Title: Effect of photobiomodulation therapy with 660 and 980 nm diode lasers on differentiation of periodontal ligament mesenchymal stem cells
    Article Snippet: .. DAPI staining (Santa Cruz, USA) was conducted at 2, 4, and 6 days. ..

    Article Title: Inhibition of HMGB1/RAGE Signaling Reduces the Incidence of Medication-Related Osteonecrosis of the Jaw (MRONJ) in Mice.
    Article Snippet: Periodontal bone loss and cemento-enamel junction (CEJ) to alveolar bone crest (ABC) distance were measured as described. (35,36) Histologic analysis Maxillae were decalcified, sectioned, and H&E stained. (37) The epithelium to alveolar crest distance, total number of osteocytic lacunae, number of empty lacunae, osteonecrotic area, and number of osteoclasts were quantified, as previously described. (37) Journal of Bone and Mineral Researchn 1778 GKOUVERIS ET AL. Immunofluorescence staining analysis Immunofluorescence staining of tissue sections were probed with rabbit polyclonal antibody against HMGB1 (Abcam, Cambridge, MA, USA; ab 18256, 1:200), mouse monoclonal antibody against RAGE (Santa Cruz Biotechnology, Dallas, TX, USA; sc 365154, 1:200) and mouse monoclonal antibody against SIRT1 (Abcam, Ab 157401 1:200). .. DAPI staining was performed to detect nuclei (Santa Cruz Biotechnology, sc-24941). ..

    Article Title: Palisade structure in intact vaccinia virions
    Article Snippet: .. When mitochondria were labeled, prior to DAPI staining, coverslips were incubated for 1 h with a mouse monoclonal antibody against Tom20 (1:500 dilution in blocking buffer, Sc-17764, Santa Cruz Biotechnology) followed by a 1 h incubation with either Alexa Fluor 647 or Alexa Fluor 488 anti-mouse secondary antibodies (1:1,000 dilution, A21235 or A21202, respectively, Thermo Fisher). .. Coverslips were imaged on a Zeiss Axioplan2 microscope equipped with a 63×/1.4 NA Plan-Achromat objective and a Photometrics Cool Snap HQ camera.

    Article Title: ETC-159, an Upstream Wnt inhibitor, Induces Tumour Necrosis via Modulation of Angiogenesis in Osteosarcoma.
    Article Snippet: FFPE sections were stained with H&E and photographed using a TS2 microscope with a DS-L4-Fi3 camera system from Nikon SBIC-NIC at Biopolis, Singapore at 40×magnification. .. For DAPI staining, the sections were stained with UltraCruz Mounting Medium (Santa Cruz, Dallas, TX, USA) and photographed using an Olympus BX61 with a CoolSNAP HQ2 camera at 40× magnification. ..

    Article Title: DUSP4 Silencing Enhances the Sensitivity of Breast Cancer Cells to Doxorubicin through the Activation of the JNK/c-Jun Signalling Pathway
    Article Snippet: .. To assess siRNA delivery to cells, the control siRNA FITC-Conjugate-A (Cat. No.: E1817, Santa Cruz) was used, and toxicity was estimated by comparing DAPI staining for negative siRNA-treated cells and control cells. .. The cells were seeded in 6/12-well plates at approximately 50% confluency and incubated for 24 h. An appropriate multiplicity of infection (MOI) of MKP-2 adenovirus (Ad-MKP-2, Cat. No. 1574, Vector Biolabs, 500 plaque forming units (pfu)/cell) was added, then incubated for 24 h at 37 °C, 5% CO 2 .

    Article Title: Molecular dynamics of PLK1 during mitosis
    Article Snippet: .. HeLa cells were synchronized by a double thymidine block and release and analyzed by immunofluorescence microscopy using anti-PLK1 antibody (clone F-8, Santa Cruz Biotechnology) and DAPI staining (blue). ..

    Immunofluorescence:

    Article Title: Telomeric lncRNA TERRA localizes to stress granules in human ALT cells
    Article Snippet: For imaging, we used Nikon AX laser scanning confocal microscope with Plan Apo λ 60x Oil objective and Nikon Eclipse Ti2 microscope equipped with the CREST Optics X-Light V2 Spinning Disk module with 100X/1.4 NA oil objective. .. For immunofluorescence, in addition to the smiFISH protocol, after the post-hybridization washes and before DAPI staining, cells were blocked for 1.5 hours with 2% BSA in PBS with 0.1% Triton-X (PBT) and subsequently incubated for 1.5 hours with primary antibodies (1:250 mouse anti-G3BP1 (sc-365338, Santa Cruz Biotechnology); 1:250 mouse anti-FUS (sc-47711, Santa Cruz Biotechnology); 1:500 mouse anti-p-histone H2A (Ser139) (05-636, Merck); 1:500 rabbit anti-TERF2IP (anti-RAP1) (NB100-292, Novus Biologicals)) washed 5 times with PBT, incubated for 1.5 hours with secondary antibodies (1:1500 goat anti-mouse conjugated with Alexa488, A28175 or 1:1500 goat anti-rabbit conjugated with Alexa647, A21245), and again washed 5 times with PBT. ..

    Article Title: Molecular dynamics of PLK1 during mitosis
    Article Snippet: .. HeLa cells were synchronized by a double thymidine block and release and analyzed by immunofluorescence microscopy using anti-PLK1 antibody (clone F-8, Santa Cruz Biotechnology) and DAPI staining (blue). ..

    Incubation:

    Article Title: Telomeric lncRNA TERRA localizes to stress granules in human ALT cells
    Article Snippet: For imaging, we used Nikon AX laser scanning confocal microscope with Plan Apo λ 60x Oil objective and Nikon Eclipse Ti2 microscope equipped with the CREST Optics X-Light V2 Spinning Disk module with 100X/1.4 NA oil objective. .. For immunofluorescence, in addition to the smiFISH protocol, after the post-hybridization washes and before DAPI staining, cells were blocked for 1.5 hours with 2% BSA in PBS with 0.1% Triton-X (PBT) and subsequently incubated for 1.5 hours with primary antibodies (1:250 mouse anti-G3BP1 (sc-365338, Santa Cruz Biotechnology); 1:250 mouse anti-FUS (sc-47711, Santa Cruz Biotechnology); 1:500 mouse anti-p-histone H2A (Ser139) (05-636, Merck); 1:500 rabbit anti-TERF2IP (anti-RAP1) (NB100-292, Novus Biologicals)) washed 5 times with PBT, incubated for 1.5 hours with secondary antibodies (1:1500 goat anti-mouse conjugated with Alexa488, A28175 or 1:1500 goat anti-rabbit conjugated with Alexa647, A21245), and again washed 5 times with PBT. ..

    Article Title: Palisade structure in intact vaccinia virions
    Article Snippet: .. When mitochondria were labeled, prior to DAPI staining, coverslips were incubated for 1 h with a mouse monoclonal antibody against Tom20 (1:500 dilution in blocking buffer, Sc-17764, Santa Cruz Biotechnology) followed by a 1 h incubation with either Alexa Fluor 647 or Alexa Fluor 488 anti-mouse secondary antibodies (1:1,000 dilution, A21235 or A21202, respectively, Thermo Fisher). .. Coverslips were imaged on a Zeiss Axioplan2 microscope equipped with a 63×/1.4 NA Plan-Achromat objective and a Photometrics Cool Snap HQ camera.

    Labeling:

    Article Title: Palisade structure in intact vaccinia virions
    Article Snippet: .. When mitochondria were labeled, prior to DAPI staining, coverslips were incubated for 1 h with a mouse monoclonal antibody against Tom20 (1:500 dilution in blocking buffer, Sc-17764, Santa Cruz Biotechnology) followed by a 1 h incubation with either Alexa Fluor 647 or Alexa Fluor 488 anti-mouse secondary antibodies (1:1,000 dilution, A21235 or A21202, respectively, Thermo Fisher). .. Coverslips were imaged on a Zeiss Axioplan2 microscope equipped with a 63×/1.4 NA Plan-Achromat objective and a Photometrics Cool Snap HQ camera.

    Blocking Assay:

    Article Title: Palisade structure in intact vaccinia virions
    Article Snippet: .. When mitochondria were labeled, prior to DAPI staining, coverslips were incubated for 1 h with a mouse monoclonal antibody against Tom20 (1:500 dilution in blocking buffer, Sc-17764, Santa Cruz Biotechnology) followed by a 1 h incubation with either Alexa Fluor 647 or Alexa Fluor 488 anti-mouse secondary antibodies (1:1,000 dilution, A21235 or A21202, respectively, Thermo Fisher). .. Coverslips were imaged on a Zeiss Axioplan2 microscope equipped with a 63×/1.4 NA Plan-Achromat objective and a Photometrics Cool Snap HQ camera.

    Article Title: Molecular dynamics of PLK1 during mitosis
    Article Snippet: .. HeLa cells were synchronized by a double thymidine block and release and analyzed by immunofluorescence microscopy using anti-PLK1 antibody (clone F-8, Santa Cruz Biotechnology) and DAPI staining (blue). ..

    Control:

    Article Title: DUSP4 Silencing Enhances the Sensitivity of Breast Cancer Cells to Doxorubicin through the Activation of the JNK/c-Jun Signalling Pathway
    Article Snippet: .. To assess siRNA delivery to cells, the control siRNA FITC-Conjugate-A (Cat. No.: E1817, Santa Cruz) was used, and toxicity was estimated by comparing DAPI staining for negative siRNA-treated cells and control cells. .. The cells were seeded in 6/12-well plates at approximately 50% confluency and incubated for 24 h. An appropriate multiplicity of infection (MOI) of MKP-2 adenovirus (Ad-MKP-2, Cat. No. 1574, Vector Biolabs, 500 plaque forming units (pfu)/cell) was added, then incubated for 24 h at 37 °C, 5% CO 2 .

    Microscopy:

    Article Title: Molecular dynamics of PLK1 during mitosis
    Article Snippet: .. HeLa cells were synchronized by a double thymidine block and release and analyzed by immunofluorescence microscopy using anti-PLK1 antibody (clone F-8, Santa Cruz Biotechnology) and DAPI staining (blue). ..



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    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were <t>counterstained</t> with <t>DAPI.</t> ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.
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    Image Search Results


    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Journal: International Journal of Nanomedicine

    Article Title: Lactoferrin-Modified Ultra-Sustained Release Propofol Nanoparticles for Multi-Pathway Therapy of Depression via Intraperitoneal Injection

    doi: 10.2147/IJN.S645818

    Figure Lengend Snippet: LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Article Snippet: The sections were subsequently counterstained with DAPI staining solution (Boster Biological Technology Co., Ltd.) for 15 min.

    Techniques: Activity Assay, Staining, Control, Immunofluorescence, Fluorescence, Tissue, Produced, Functional Assay, Marker, Expressing